Is this not a standard C# question?
// in reality your DataTable would be populated from somewhere?
DataTable myDataTable = new DataTable();
ListBox myListBox = new ListBox();
// apply data source and bind it
myListBox.DataSource = myDataTable;
myListBox.DataBind();
regards
Martin Hatch
MCPD SharePoint 2010 | .Net Web Development
MCTS WSS 3.0 | MOSS 2007
Visit my Blog - www.martinhatch.com