Well, this forum and group is for MS Access, and not for SQLite, and not for Visual Studio.
So, few if anyone here will be able to help you – unless your question is/was about MS Access.
However, since that file is “in use” and locked, obviously VS is not letting you build + publish your project.
I would exit VS, and do NOT test run the application but then try and build + publish. If you are testing software, then often “some” of the files will become “in use” by the application software.
So starting with a clean start of VS, and not actually running/testing the project would be something to try.
However, you only going to get “long shot” answers here, since you’re posting in the wrong group.
Regards,
Albert D. Kallal (Access MVP, 2003-2017)
Edmonton, Alberta Canada