File Share witness is part of your WSFC creation. You can use Quorum disk or file share.
To build WSFC you need minimum of 2 nodes and a domain controller.
You can have domain controller on one of the nodes but it is not recommended.
So you need minimum of three servers one for DC and two for nodes.
Only two nodes can be part of the SQL AlwaysOn .
YOu are required to install SQL standard installation on each node and enable always on feature.